Genetic polymorphisms at the TNF locus in Chinese Han population
- VernacularTitle:中国湖北地区汉族人群TNF遗传多态性研究
- Author:
Baoying FEI
;
Changsheng DENG
;
Bing XIA
;
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor Genetic polymorphism Polymerase chain reaction
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the distribution of TNF microsatellite polymorphisms in Chinese Han population of Hubei province Methods:DNA samples were extracted from 164 unrelated healthy individuals’EDTA blood TNF microsatellite alleles were typed using PCR technique,followed by High Voltage denaturing PAGE,with silver staining At the same time,the PCR products were cloned and sequenced Results:Detected seven alleles and eleven kinds of genotypes at the TNFb locus;four alleles and seven kinds of genotypes at the TNFe locus The polymorphism information contents (PIC) were 0 67 and 0 33 respectively No deviation from Hardy Weinberg equilibrium were observed Statistical analysis showed,the distribution of TNFb and TNFe alleles in Chinese Han population were significantly different from that in European or in American Caucasian(P
