Cloning of HLA-A~*0201 and its transient expression on COS-7 cells
- VernacularTitle:HLA-A0~*201的cDNA克隆及在COS-7细胞上的瞬时表达
- Author:
Qiang YOU
;
Hailiang GE
;
Xiaoren ZHANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
HLA A *0201 Gene cloning COS 7 cell Transient expression
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone HLA A *0201 cDNA and express it transiently on COS 7 cells.Methods:HLA A cDNA isolated from the HLA A *0201 positive lymphocytes by using RT PCR was cloned into pBluescript II SK vector directionally.After the sequence was confirmed,the cDNA was inserted into mammalian expression vector pcDNA3.The recombinant vector was transfected into COS 7 cells by cation liposome.The transient expression on the cells was measured by flow cyteometer.Results:The cDNA was identical with HLA A *0201 cDNA published on Genebank.Determined by flow cytemeter,the expressing rate was recorded for 57%.Conclusion:We have cloned HLA A *0201 successfully and expressed it transiently on COS 7 cell,which would be potentially useful in research on killing tumor restricted by HLA A2.
