THE EFFECT OF DEXAMETHASONE IN DIFFERENTIATING HUMAN GLIOMA CELL LINE
- VernacularTitle:地塞米松对人胶质瘤细胞系诱导分化作用的初步观察
- Author:
Yilu GAO
;
Xiangling TAN
;
Mei LIU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Glioma;
Differentiation
- From:
Cancer Research and Clinic
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of dexamethasone in differentiating human glioma cell line .Methods: The human glioma cell line was incubated with l mg/L dexamethasone in 1640 culture medium with 10% vitulary serum for 48 hours. The cell form was observed by contrapositive microscope,and PHA was used to induce the agglutination of these cells.Mitotic index and AgNOR amount was counted. The expression of GFAP was detected by immunocytochemisty.Rusults: SHG 44 cells incubated with dexamethasone adhered to plate firmly and its shape became astroid .The agglutination degree and mitotic index decreased significantly .The Ag NOR was atrophied and its amount decreased.The Immunocytochemistry showed the content of GFAP increased sign ficantly.Conclusion: Dexamethasone plays a role in differentiating human glioma cell.
