Biological effect of recognizing hepatoma antigen TCRV?7 subfamily after cloning and transgene
- VernacularTitle:识别肝癌抗原的TCRV?7亚家族克隆转基因后的生物学作用
- Author:
Weichun CHEN
;
Yuexia LIN
;
Shulin HUANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
TCRV?7 gene Transgene Apoptosis
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the biological effects of TCR to hepatoma cell by transfection V?7 to lymphocytes. Methods:TCRV?7 gene was amplified by RT PCR and cloned to expression vector pLXSN. The recombinant was transferred into lymphocytes by Lipfectin Reagent transfection,then the lymphocytes were co cultured with hepatoma cells.The phenotype of lymphocytes was detected on the Flow Cytometry and the ultrastructure of the hepatoma cells was showed by electronic microscope.Results:The lymphocyte amount with TCRV?7 expressing in those being transfected was much more than those no transfection.Apoptosis appeared in the hepatoma cells.Conclusion:TCRV?7 subfamily can recognize hepatoma antigen and stimulate T cell.
