Isolation and purification of basement membrane zone mono-specific autoantibodies by spot affinity purified method
- VernacularTitle:斑点亲和纯化法分离纯化表皮下大疱病单特异性基底膜带自身抗体
- Author:
Zaixing WANG
;
Xuejun ZHANG
;
Peiguang WANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Spot affinity purified method Subepidermal autoimmune blistering disases Basement membrane zone autoantibody
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the value of spot affinity purified method in the isolation and purification of mono specific basement membrane zone autoantibodies(BMZ Ab).Methods:Utilizing human dermoepidermal antigen transmitted on NC membrane,We purified 3 cases with mono specific BMZ Ab in sera of SABD patients which was oppositive by immunoblotting.Results:The BMZ Ab purified by this method could specifically combine its target antigen.Conclusion:Spot affinity purified method could be widely used in the isolation and purification of autoantibodies produced by mixed micro antigen.
