Immunogenicity of dengue virus E antigen gene in BALB/c mice
- VernacularTitle:登革病毒E蛋白抗原基因肌肉注射诱导小鼠免疫应答的初步研究
- Author:
Bangxing HONG
;
Lifang JIANG
;
Xin ZHANG
;
Huiyu GUO
;
- Publication Type:Journal Article
- Keywords:
Dengue virus Gene vaccine Immune respon
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the possibility of dengue virus E gene vaccine.Methods:The recombinant eukaryotic expression plasmid pcDNA3 E was first transfected into NIH3T3 cells by lipofectin SDS PAGE and Western blotting analyzed the expression of E gene Then the recombinant plasmid was intramuscularly injected to BALB/c mice,and the specific humoral and cellular immunity were tested Results:The recombinant plasmid DNA could induce specific immune reactions and the immune response could last a long time Conclusion:The dengue virus E gene vaccine could induce specific immune reaction,which might have provided some material and new experimential data for the further study of dengue vaccines
