All-trans retinoic acid sensitizes human medulloblastoma cells Med-3 to anti-Fas antibody mediated apoptosis
- VernacularTitle:抗Fas抗体诱导维甲酸分化后的髓母细胞瘤细胞凋亡
- Author:
Li GUO
;
Jia LIU
;
Junwei LI
;
Al ET
- Publication Type:Journal Article
- Keywords:
All trans retinoic acid Medulloblastoma Differentiation Anti Fas antibody Apoptosi
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:It was aimed to highlight the pro apoptotic significance and immunotherapeutic value of the membrane type Fas(mFas)up regulated in all trans retinoic acid(RA)differentiated medulloblastoma cells Med 3.Methods:Med 3 cells were treated by 10 ?mol/L RA for 0,24,48 or 72 h,followed by the supplementation of anti Fas antibody in the final concentration of 25,50,100,200 or 400 ng/ml.The cell morphology ,growth and death pattern(s)were evaluated by multiple approaches.Results:RA could promote cell differentiation and mFas expression but not induce apoptosis.Anti Fas antibody alone had no biological effect on Med 3 cells but when combined with 10 ?mol/L RA,showed pro apoptotic activity in a dose related fashion.The best apoptotic induction could be achieved in the cell population pretreated by RA for 48 h.Conclusion:The RA enhanced apoptotic susceptibility of Med 3 cells is closely associated with mFas up regulation.The chemical activation of Fas suicide system may be a novel regimen for medulloblastomas.
