Effects of Cadmium on DNA Damage in Rat Liver Cells,in Vivo and in Vitro Studies With Single Cell Gel electrophoresis
- VernacularTitle:应用单细胞凝胶电泳研究镉对大鼠肝细胞DNA损伤的影响
- Author:
Rian YU
;
Xuemin CHEN
;
- Publication Type:Journal Article
- Keywords:
Cadmium;
DNA damage;
Single cell gel electrophoresis
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the effects of cadmium chloride on DNA damage of rat liver cells in vivo and in vitro Single cell gel electrophoresis or Comet assay was used At the concentration of 2 185 ?mol/L, 4 375 ?mol/L, 8 75 ?mol/L, 17 50 ?mol/L, 35 00 ?mol/L, cadmium chloride could induce DNA damage of rat liver cells in vitro, and at the doses of 5 ?mol/kg, 10 ?mol/kg, 20 ?mol/kg, cadmium chloride could induce DNA damage of rat liver cells in vivo respectively The in vivo and in vitro results also showed the obvious dose response relationship between the rates of Comet cells and the doses of cadmium chloride [Conclusion]The research infered that at certain dose, cadmium could induce DNA damage and had toxic effects on rat liver cells
