Inhibition of ICAM-1 expression in renal tubular epithelial cells with ICAM-1 antisense oligodeoxynucleotide
- VernacularTitle:ICAM-1反义寡核苷酸对小鼠肾小管上皮细胞ICAM-1表达的影响
- Author:
Qingli CHENG
;
Xiangmei CHEN
;
Bo FU
;
Al ET
- Publication Type:Journal Article
- Keywords:
ICAM 1 Antisense Epithelial cell Transfection Kidney
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The blocking effects in ICAM 1 antisense oligodeoxynucleotide (ASON) on the ICAM 1 expression of renal tubular epithelial cell was investigated to probe the potential applicability of the ASON drug in renal tubulointerstitial diseases.Methods:The mouse ICAM 1 ASON was transducted into the mouse renal tubular epithelial cells with the presence of liposome DOTAP.Another oligodeoxynucleotide,which has the same base composition as the ICAM 1 ASON but a different sequence,was also transfected into the cells as a control.ICAM 1 expression of the epithelial cells was induced by interleukin 1.The changes of ICAM 1 protein expression by the cells were measured by immunohistochemistic stainging with anti ICAM 1 antibody.ICAM 1 mRNA expression of the epithelial cells was examined by RT PCR and Northern blotting methods.Results:①ICAM 1 ASON inhibited the ICAM 1 and ICAM 1 mRNA expressions of the epithelial cells induced by IL 1 at dosages of both 100 and 200 nmol/L.②The control oligodeoxynucleotide has no effect on the ICAM 1 expression of the epithelial cells.Conclusion:These data demonstrate that ICAM 1 ASON can selectively inhibit the ICAM 1 expression of the cells.It suggests that the ICAM 1 ASON might be applied in treatment of renal tubular interstitial diseases.
