THE INHIBITION OF CELL PROLIFERATION IN CARCINOMA OF LARGE INTESTINE BY TRANSFORMING GROWTH FACTOR-ALPHA
- VernacularTitle:TGF?反义寡聚核苷酸抑制大肠癌细胞株的增殖
- Author:
Shumin WANG
;
Jinsheng WU
;
Xiu YAO
;
Al ET
- Publication Type:Journal Article
- Keywords:
TGFa;
HR8348 cell line;
Anti-sense;
Oligodeoxynucleotides
- From:
Cancer Research and Clinic
1999;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibition of transforming growth factor-alpha(TGFa) anti-senseoligodeoxynucleotides on the malignant proliferation of HR8348 cell line. Methods Using the TGFa anti-sense oligodeoxynucleotides, composed of 23 and indifferent oligodeoxynucleotides, to affect the HR8348cell. By observing the cell growth inhibiton, 3H-TdR incorporation,mRNA hybridization and the cell cycleanalysis to identify the inhibiting effects of TGFa anti-sense oligodeoxynucleotides and its mechanism.Results TGFa anti-sense oligodeoxynucleotides can Inhibit the proliferation of HR8348 cell, DNA0synthesis,mRNA expression,and defer the transition period of G,/G, phase to S phase. Conclusion TGFaanti-sense oligodeoxynucleotides can inhibit the malignant proliferation of HR8348 cell effectively.
