Search for Gene Expression in Cervical Squamous Cell Carcinoma using GeneFishing(TM) DEG PCR Technique.
- Author:
Hee Jeong YU
1
;
Byung Hoon KIM
;
Su Mi BAE
;
Young Wook KIM
;
Joon Mo LEE
;
Sung Eun NAMKOONG
;
Heung Jea CHUN
;
Kye Hyun NAM
;
Young Lae CHO
;
Chong Kook KIM
;
Byoung Don HAN
;
Woong Shick AHN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea. hbk8392@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Cervical squamous cell carcinoma;
GeneFishing;
Differential expressed genes;
Human papilloma virus
- MeSH:
Biopsy;
Calgranulin A;
Carcinoma, Squamous Cell*;
Clusterin;
Collagen;
Female;
Galectin 1;
Gene Expression*;
Genomics;
Gynecology;
Humans;
Obstetrics;
Oncogenes;
Polymerase Chain Reaction*;
RNA;
RNA Precursors;
Transcriptome;
Uterine Cervical Neoplasms
- From:Korean Journal of Obstetrics and Gynecology
2005;48(3):617-627
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The aim of this study was to investigate the gene expression profiles using GeneFishing(TM) DEG kit in Korean women with cervical squamous cell carcinoma. METHODS: Cervical cancer biopsies were obtained from patients at the Department of Obstetrics and Gynecology, St. Mary's hodpital. In this study, we used a common reference that was mixed with an equal amount of RNA extracted from non-cervical cancer patients. The profiles of expression genes between cervical normal and squamous cell carcinoma tissue were identified using GeneFishing(TM) DEG Kit and screened by BLAST search. RESULTS: Almost 100 differential expressed genes were identified in universal control and cervical squamous cell carcinoma, 53 of differential expressed genes, up-regulated expression of 32 and 21 down-regulated expression was sequenced. Up-regulated genes were calcylin, calgranulin A, TRK oncogene, HLC5, fibrillarin, collagene type I alpha1 etc. and down-regulated genes were galectin 1, PRP8 pre-mRNA precessing factor 8 homology, clusterin etc. CONCLUSION: We identified gene expression profile in cervical squamous cell carcinoma using GeneFishing(TM) Kit in Korean women. The functional genomics of these genes should be further studied.
