THE EXPERIMENTAL STUDY ON CULTURE OF CHONDROCYTES SEEDED ONTO POLYGLYCOLIC ACID SCAFFOLDINGS COATING WITH DIFFERENT MATERIALS
- VernacularTitle:包埋后的聚羟基乙酸与软骨细胞体外培养实验研究
- Author:
Yanchun LIU
;
Wei WANG
;
Yilin CHAO
;
Qingxin SHANG
;
Han ZHONG
;
- Publication Type:Journal Article
- Keywords:
Lecithin;
Poly-l-lysine;
Polyglycolic acid;
Polylatic acid;
Chondrocytes;
Culture
- From:
Chinese Journal of Microsurgery
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This experiment aims to find a appropriate scaffold for Tissue Engineer- ing.Methods:The chondrocytes were cultured when they were seeded onto PGA+PLA scaffolds coat- ing with Lecithin(LEC)and Poly-l-lysine(PLYS)together and respectively.With light microscope and scanning electron microscope,the observation of the scaffolds in hydrophilia and adsorptivity to chondrocytes and the function of the cells was made.Results:The PGA scaffolds coating with LEC and PLYS have better hydrophilia and adsorptivity to the cells;on which the chodrocytes produce more ma- trices.Conclusion:LEC can chang the hydrophilia of the scaffolds;while PLYS can strengthen the ad- sorptivity of the scaffolds;the PGA coating with LEC and PLYS is an ideal scaffold in Tissue Engineer- ing.
