In vitro inhibition of trichosanthin-monoclonal antibody conjugate on human melanoma cells
- VernacularTitle:天花粉毒蛋白免疫毒素对黑色素瘤细胞的体外抑制
- Author:
Ru-Ping ZHANG
;
Chi-Jie XU
;
Hui-Ting CAO
;
Rui-Hua JI
;
Zu-Chuan ZHANG
;
- Publication Type:Journal Article
- Keywords:
Trichosanthin;
Anti-human melanoma monoclonal antibody;
Immunotoxin;
Human melanoma cell
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
We have constructed an immunotoxin(Ng76-TCS),which was composed of a monoclonalantibody directed against human melanoma and trichosanthin(TCS)——a single chain ribosomeinactivating protein.The cultured human melanoma cells(M21)were inhibited effectively byNg 76-TCS.The cytotoxicity of Ng76-TCS to M21 cells was 2,000-fold higher than that of free TCS and Ng76 mixture.A conjugate,which was prepared with normal mice immunoglobulinand TCS(NIgG-TCS),was 160-fold less cytotoxic to M21 cells.Meanwhile Ng76-TCS was125-fold less cytotoxic to nontarget cells Hela.These results showed that the immunotoxinNg76-TCS was a potent and specific anti-human melanoma agent.
