Preparation of anti-gastric cancer monoclonal antibody with daunorubicin methotrexate conjugate and its cytotoxiciy to gastric cancer cells.
- VernacularTitle:胃癌单抗与柔红霉素和氨甲喋呤交联物的制备及其细胞毒作用
- Author:
Pu-Jun GAO
;
Xiao-Lin GUO
;
Guo-Pei SONG
;
Yun-Feng PIAO
;
- Publication Type:Journal Article
- Keywords:
McAb;
Gastric cancer;
DNR;
MTX;
Targeted treatment
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
It is first time to use dextren T-40 oxidative method to conjugate anti-gastric cancer mono-colonal antibody(McAb)with anti-tumor medicines of daunorubicin(DNR)and methotrexate(MTX)together.Cytotoxicity of conjugates was measured by MTT method and ~3H-TdR incor-poration method respectively.Both sensitivity is similar.The results have showed that this conju-gate exhibited selective cytotoxicity on human gastric cancer cells in vitro.
