Preparation and identification of monoclonal antibodies against cell receptors of acute hemorrhagic conjunctivitis viruses
- VernacularTitle:抗急性出血性结膜炎病毒受体单克隆抗体的制备与鉴定
- Author:
Li-Na LI
;
Gui-Fan MU
;
- Publication Type:Journal Article
- Keywords:
acute hemorrhagic conjunctivitis viruses;
cellular;
receptor;
monoclonal antibody
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To prepare monoclonal antibodies for blocking acute hemorrhagic conjunctivitis (AHC)viruses,BALB/C mice were immunized with Hela cells.The spleen of immunemouse was re-moved,and spleen cells were fused with SP2/0 cells.The antibodies against cell receptors wereselected by cell protection assay.Four cell strains secreted McAbs designated R7.R8.R16 andR24 were screened to be able to block cellular receptors and inhibit the infection of Ev70 as wellas CA24v on Hela cell.These results demonstrate that the receptors for Ev70 and CA24v are si-miliar.Indirect immunofluorescence test with the four McAbs showed definite fluorescence onliving Hela cells,which the intensity was concordance with the mean intensity resulted fromflowcytometer.Positive reaction on fixed Hela cells were only observed with R7 and R8.
