Expression Levels of IL-6 mRNA in PBMNCs from Patients with IDDM,NIDDM and Normals by RT-PCR Procedure
- VernacularTitle:应用RT—PCR技术检测糖尿病人外周血单个核细胞中IL—6mRNA的表达水平
- Author:
Wei HUANG
;
Deng-Shun WANG
;
Xin-Yuan LI
;
Wei-Zheng WU
;
Gui-Cheng NI
;
- Publication Type:Journal Article
- Keywords:
IL-6;
mRNA;
IDDM;
NIDDM;
PCR
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Whether or not an abormal expression of IL-6 mRNA in PBMNCs from IDDM patientswas examined using a hihgly sensitive,specific and semiquantitative protocal,i.e.reverse tran-scription and polymerase chain reaction (RT-PCR).The relative levels of IL-6mRNA in PBM-NCs from 12 early IDDMpatients (8.20?3.85yr),29 newly diagnosed NIDDM patients(54.85?9.12yr)23 normal childrens (8.20?3.26yr) and 12 normal adults (31.92?11.22yr)weredetermined.Significantly high expresion levels of IL-6 mRNA were found in PBMNCs from pa-tients with IDDM (P
