EFFECTS OF TRH AND INTERLEUKIN-1? ON CYTOSOLIC FREE CALCIUM MOBILIZATION IN RAT GH_3 PITUITARY CULTURED IN VITRO

VernacularTitle:TRH、IL-1?对大鼠垂体GH_3细胞游离钙影响的体外研究
Author: Xiao-Dong WANG ;
Publication Type:Journal Article
Keywords: Thyrotropjn-releasing hormone Interleukin-1? GH_2 cell Cytosolic Ca~(++)
From: Chinese Journal of Endocrinology and Metabolism 1985;0(01):-
CountryChina
Language:Chinese
Abstract: The effects of TRH and recombinant human IL-1? on cytosolic free calcium mobilization in rat GH, pituitary cells cultured in vitro was studied spectrofluorometrically using Fura-2 as Ga++ indicator. The results showed that TRH 10-7～10-9mol/L stimulated the cytosolic Ca++mobilization in a dose-dependent manner. IL-1 alone in the concentration ranging from 0.01 to 10 U/ml did not increase the intracellular Ca++ level. 1- and 3-minute pretreatment of the GH3 cells with IL-1? 0.1 U/ml significantly inhibited TRH-induced Ca++ mobilization, whereas such suppressive effects were significantly diminished when IL-1/3 and TRH were added at the same time. These findings suggest that Ca++ may signal a TRH effect in pituitary cells and IL-1? influences pituitary function by inhibiting Ca++ mobilization induced by TRH.