MYO minisatellite DNA probe and sub—cloning of MYO fragment
- VernacularTitle:MYO小卫星DNA探针及其片段亚克隆
- Author:
Yu-Xin LI
;
Gui-Zhen YANG
;
- Publication Type:Journal Article
- Keywords:
Minisatellite DNA;
DNA recombinant techniques;
Sub-clone
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The initial study of the tumour DNA fingerprints using MYO minisatellite DNA probe wascarried out,and then,by means of DNA recombinant techniques,the fragment of MYO min-isatellite DNA probe obtained from plasmid pUC19-MYO was inserted into plasmid pGEM-4Z containing RNA polymerase promotor,thus a sub-clone refferred as pGEM-4Z-MYOwas constucted.That made an offer of the conditions of preparing RNA probe in order to in-cerase the sensitivities of DNA fingerprinting and laid a foundation for raised the efficiency of de-tecting the polymorphism of the minisatellite DNA.
