Purification of recombinant human IE—2 by McAb affinity chromatography
- VernacularTitle:单克隆抗体亲和层析法提纯人重组IL—2
- Author:
Xiao-Li WANG
;
Ben XU
;
Lan ZHENG
;
Ying-Hua XIE
;
Xian-Tao KONG
;
- Publication Type:Journal Article
- Keywords:
McAb affinity chromatography;
purification;
rhIL-2
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
For purifying recombinant human IL—2 (rhIL—2),the columns of immunoabsorptionwere prepared with 4 anti—IL—2 McAb (9B12,9F5,9B2 and 8H7) purified by caprylic acid.Although 4 McAbs differ as regards their antigen—antibody binding characteristics,all they canserve as effective immnoabsorbents,provided optimum condition was adopted.The recoveryrate of 9B12,9F5,8H7 and 9B2 columns were 49.2%,37.5%,31.5% and 18.8% respec-tively.The purity of rhIL—2 obtained was more than 95% and biological activity remainedhigher.
