Establishment of a sandwich ELISA for measuring urokinase(UK)antigen
- VernacularTitle:测定尿激酶含量夹心法 ELISA 的建立
- Author:
Hao DING
;
Xiaoli WU
;
Yunsong ZHU
;
Houyan SONG
- Publication Type:Journal Article
- Keywords:
Urokinase(UK);
Sandwich ELISA;
Monoclonal antibody(McAb)
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A sandwich ELISA for the measurement of urokinase(UK)antigen was developed based on anti—UK monoclonal antibodies(McAbs)against two non—overlapping epitopes.The lower limit of sensitivity of the assay was 0.15ng/ml.Coefficients of variation of the assay at physilogi-cal levels of UK were 4.3 percent within assaysw and 8.7 percent between assays.The recovery of added UK was about 98 percent.Culture media of some human malignant cell lines contains more UK than that of normal cell lines measured by our assay.The ELISA was used to measurethe concentration of UK in plasma from 82 healthy donors.The mean value for the healthy donors was 1.31?60.6ng/ml of UK in plasma.
