The study on TLSF_(JM)mRNA translation in Xenopus Lavevis Oocytes
- VernacularTitle:非洲爪蛙卵母细胞系统 TLSF_(JM)mRNA 体外翻译研究
- Author:
Haibing XIA
;
Boquan JIN
;
- Publication Type:Journal Article
- Keywords:
Inhibitory factor;
RNA;
Microinjection;
Translation in vitro
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Total RNA from JM cell lines secreting T leukmia derived suppressor factor was isolated using single—step method of acid guanidium followed by oligo—d(T)—cellulose affinity chromotogra-phy for purification of mRNA.TLSF_(JM)mRNA was mieroinjected into Xenopus Laevis Oocytes.The inhibitory activity in Xenopus Laevis Oocytes culture supernatant or lysate was detected by PHA plus IL—2 induced—mouse thymocyte proliferation assay The results showed that Xeno-pus Laevis Oocytes could efficiently translate exogenous mRNA,the trans ated products mainly existed inside oocytes and approximately2% of the products was secreted into the culture super-natant.Success in TLSF_(JM)mRNA translation in vitro might provide the basis for establishment of cDNA library and TLSF_(JM)gene cloning and expression.
