~3H-THYMIDINE UPTAKE ASSAY OF FRTL5 CELLSI THE INFLUENCE OF CELL FACTORS
- VernacularTitle:甲状腺细胞的~3H-TdR摄取量测定的细胞因素影响
- Author:
Shixin JIN
;
Hui GAO
;
- Publication Type:Journal Article
- Keywords:
FRTLS Cell;
Frozen cell;
~3H-Thymidine Uptake
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The amount of ~3H-TdR incorporation into D?A of FRTL5 cells was expressed as CPM/?gD?A. THe responsibity of FRTL5 cells to stimulators such as TSH was expressed as % against thJ control CPM/?gDNA of the experimental group divided by that of the control. The responsibilities of FRTL5 cells which were recovered after being fr-o zen under-180℃ for a long time, to TSH 200?g/ml were 2741% and 3028% respectively, and the responsibilities of cells cultured in TSH media continuously for a long time, to TSH 200?u/ml and 2000?u/ml were 736% and 719% respectively. There was statistically significant differences between the two groups of the cells mentioned above. This showed that freezing FRTL5 Cells for a long time Such as 2 months was able to recover the decreased responsibility of the Cells due to a long continuous TSH culture.
