The Establishment of Hybridoma Cell Lines Secreting Antikeratin Monoclonal Antibodies and Their Preliminary Characteristics
- VernacularTitle:分泌抗人角蛋白单抗杂交瘤细胞株的建立及其抗体的初步鉴定
- Author:
Jun HU
;
Weike FAN
;
- Publication Type:Journal Article
- Keywords:
Keratin;
Hybridoma cell lines;
Monoclonal antibody.
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The two hybridoma cell lines secreting antikeratin monoclonal antibodies (McAb)were obtained from fusion between NS_1 myelema cells and spleen cells of BALB/cmice immunized with keratin prepared from human callus.The two cell lines(AF_2and AF_5)have a chromosome number of 97 and 96 respectively.By immunodiffusion,both AF_2 and AF_5 McAb belong to mouse IgG_1 subclass.They have been successivelypassaged for over six months in vitro,and can grow well in the peritoneal fluid ofBALB/c mice,both produce high titer of specific antibodies. To determine their specificity with keratin of epithelia,AF_2 and AF_2 McAbwere used to stain various human normal and neoplastic tissue sections by the ABC(Avidin-Biotin peroxidase complex)immunohistochemical techniques.The results showthat AF_2 and AF_5 McAb can stain a series of normal epithelial tissues(in epidermis,positive for cells above the basal layer)and epithelial tumor tissues.No staining wereobserved in nonepithelial normal and tumor tissues.These results suggest that the twoMcAb are quite specific for a definite number of cytokeratins and promise to be use-ful in the differential diagnosis of tumors.
