Immunological Assay for Duck Hepatitis B Virus and Its Uneven Geographic Distribution
- VernacularTitle:鸭乙型肝炎病毒的免疫学检测方法及区域分布差异
- Author:
Chenggang SUN
;
Weifa YE
;
- Publication Type:Journal Article
- Keywords:
Duck hepatitis B virus;
Duck hepatitis B surface antigen;
Density gradient centrifugation;
Reverse passive hemogglutination assay;
Geographic distribution of duck hepatitis B virus
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The duck hepatitis B surface antigen(DHBsAg)particles were purified from the s-era of ducks by sucrose rate zonal and KBr isopycnic centrifugation.Analysis of DH-BsAg by SDS-PAGE revealed the major polypeptide to be 18Kd.The anti-DHBsAg-serum was made by immunizing cavy or rabbit and the anti-DHBsA-IgG was purifiedby DEAE-Sephadex A50.A simple,specific and sensitive Reverse Passive Hemgglutin-ation Assay for DHBsAg have been established with the method of Glutaraldehyde T-wo-step Procedure.The sera of ducks from three districts determined by RPHA andelectromicroscopy showed that the geographic distribution of DHBV is uneven and thearea of higher incidence of hepatitis B is also that of duck DHBV infection.So.therewould be other environmental facts influencing the uneven distribution of DHBV,andprobably that of HBV.
