Production and characterzation of mcAb to murine astrocyte and some biological properties of astrocyte product
- VernacularTitle:鼠脑星形细胞单抗及星形细胞产物的生物学特性研究
- Author:
Yi LI
;
Guizhen YANG
;
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Using hybridoma technique,17 hybridoma cell lines against astrocyte were ob-tained.The screening test of McAb showed:(1)Using immune enzymatic histochemistryfixed cell staining method all of 17 McAb reacted with astrocyte,(2) Using immuneenzymatic histochemistry viable cell staining method,eight of seventeen McAb react-ed with astrocyte.As to biological properties of McAb results were as follows:(1)Seven of eight McAb that showed positive reaction in viable staining assay have di-fferent degree of cytotoxicity to astrocyte,thymocyte and spleen cell in presence ofcomplement,one of eight has specific cytotoxicity only to astrocyte.(2) McAb(2C)possesses blocking effect on astrocyte,thymocyte and spleen cell proliferation respon-sive to various kind of mitogen.Furthmore,it has been found LPS may promote theproliferation of astrocytes and secreting interleukin 1(IL-1)-like substance This IL-1like substance seems to have a mutiple biological activities,i.e:(1)Thymocyte prolife-ration.(2) Fibroblast(L929 cell) proliferation and(3) Endogenous pyrogen activities.
