MeaSurement of Relative Affinity and Specificity of Monoclonal Antibodies
- VernacularTitle:单克隆抗体相对亲和力和特异性的测定
- Author:
Chun-Hai HAO
;
Yuan-Yao CHEN
;
- Publication Type:Journal Article
- Keywords:
Affinity;
Specificity;
Monoclonal antibody;
Adenovirus type 7;
ELISA
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To measure the relative affinity and type Specificity of neutralizing monoclonal antibodies against the hexon antigen of adenovirus type 7,a simple ELISA double antibody binding system has been developed.By this method,ralative affinity of nine MAbs was estimated from the antibody concentrations at approximately 50% of plateau binding and were ranked.The theoretical basis for this method was discussed. Hybridomas secreting antibodies of desired affinity can be selected at an early stage after fussion by measuring relative affinity of hybridomas supernatants.The type specificity was judged by the difference between end-point concentration against adeno- virus type 7 and type 3.And the relation between affinity and specificity of MAbs was discussed.
