Study on the Immune regulation of anti-idiotypic antibodies against anti-HBs in HBV infection——一、The production of anti idiotypic antibodies against anti HBs
- VernacularTitle:抗个体型抗体对HBV感染的免疫调节作用——抗-HBs的抗个体型抗体的制备和鉴定
- Author:
Wenxiang JIA
;
Zongrong ZHAO
;
Meiying CAI
;
Jiawan MOU
;
Daoruo WANG
;
- Publication Type:Journal Article
- Keywords:
anti-HBs anti-Idiotype antibodies identification
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The anti-idiotypic antibodies Specific for anti-HBs were prepared by Immunizing New Zealand white rabbits with anti-HBs according to the method of Kennedy. The serum was first purified through IgG of normal human-Sepharose 4B affinity Column.The anti-idiotypic Specificity of the antibodies obtained was identified with ELISA or RIA inhibition test.The resultshowed that the anti-idiotypic antibodies were directed at the idiotypic determinants on anti-HBs,and no cross reaction between anti-idiotypic antibodies and normal human IgG.
