Hb-Enzyme Release Assay for Macrophage-Mediated Aptibody-Dependent Cellular Cytotoxicity
- VernacularTitle:血红蛋白酶释放法检测巨噬细胞介导的抗体依赖性细胞毒效应
- Author:
Sheng WEI
;
Zu-Qiong CHEN
;
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
An improved assay for quantitation of murine macrophage-mediated antibody-dependent cellular cytotoxicity (MMADCC) by hemoglobin-enzyme release assay (Hb-ERA) has been developed The method is based on the coloration measurement by spectrophotometer,because hemoglobin has peroxidase activity capable of catalyzing the oxidation of o-phenylenediamine and producing color reaction. This method was applied to demonstrate variation of MMADCC activity with varying effector:target ratio,incubation time,antibody concentration and macrophage in different stage of activation. The method provides advantages of(l)elimination of the need for expensive and hazardous radioactive materials,(2)relative ease and rapidity, (3)sensitiyity and reproducibility.
