Study of Reagents of Biotin-Avidin System and Their Applications Ⅴ.The Detection of Antibodies to Cell Surface Antigens with ABC-ELISA
- VernacularTitle:生物素-亲和素系统试剂及其应用的研究 Ⅴ?用ABC-ELISA检测细胞表面抗原的抗体
- Author:
Shaokang LI
;
Gusheng ZHANG
;
Yun ZHOU
;
Yuejian WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Two reaction systems have been observed to ascertain the capability of ABC-ELISA on the detection of antibodies against particulate antigens.Mouse thymucytes as the antigens were coated on the enzyme immunoassay plate for one system,and rabbit anti- mouse brain serum was titrated.The titer of the serum in ABC-ELISA was 1:12,800, about 40-fold higher than that in complement dependent cytotoxicity assay.In the other system,the plate was coated with cultured human leukemia cells of T lymphocyte origin(Cem-T)and B cell origin(Daudi),where the ascites induced by an anti-human lymphocyte hybridoma was titrated.The results showed that ABC-ELISA was 8-16 times sensitive than the standard ELISA,while the specificity and reliability of ABC- ELISA seemed satisfectory.The experi mental condition that might reduce nonspeci- ficity of the reaction to a certain degree was discussed.
