Natural-killer cell(NK)activity as determined by ~(51)Cr-and ~(125)I-UdR release assays a comparison
- VernacularTitle:天然杀伤(NK)细胞活性的测定——~(125)I—UdR及~(51)Cr释放试验的比较
- Author:
Wei SHI
;
Yi-Yuan WU
;
You-Hui ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Target cells K562 were labeled using two different isotopes,~(51)Cr and ~(125)I-UdR,for detecting NK activity of peripheral blood mononuclear cells in normal subjects.The NK activity was higher in ~(51)Cr release assay in comparison with ~(125)I-UdR release assay. After six-hour incubation,the percentage release of ~(51)Cr was around 60% whereas that of ~(125)I-UdR was only 30% in 20 hours. ~(125)I-UdR release could be enhanced by trypsin treatment.
