The Interaction between Interin-1? and Fibroblast Proliferation,MMP-2,MMP-9 in Patients with Diabetic Foot Ulcers
- VernacularTitle:糖尿病足部溃疡患者白介素-1?对成纤维细胞增殖及基质金属蛋白酶-2和基质金属蛋白酶-9的影响
- Author:
Zheng-Hua XIAO
;
Zheng-Jun ZHANG
;
Qian ZHOU
- Publication Type:Journal Article
- Keywords:
Chronic diabetic foot ulcer;
IL-1?;
Matrix metalloproteinase;
Fibroblast
- From:
Chinese Journal of Prevention and Control of Chronic Diseases
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Abstract:Objective To inquire into the reason that chronic diabetic foot ulcer(CDF) was difficult to be cured by clarifying their interaction between interin-1?(IL-1?) and fibroblast proliferation,the activity and protein expression of matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9)in fibroblasts from CDF.Method Fibroblast collected from the edge area of the diabetic foot ulcer was cultured for 4-6 generations,and added in with different concentration of IL-1? at the 24 hr,48 hr and 72 hr,then the effect of IL-1? on the activity and expression of MMP-2 and MMP-9 in fibroblasts were observed by methods of zymography and Western blott.Results There was a significant difference of IL-1? between CDF and acute diabetic lesions(ADL);IL-1? in 0.5 ng/ml-5 ng/ml could promote fibroblast proliferation obviously,but weakened gradually with increasing of the IL-1?,but no affection on fibroblast proliferation when IL-1? was in 50 ng/ml,and inhabited fibroblast proliferation over 50 ng/ml of IL-1?;IL-1? in 50 ng/ml increased the activity and protein expression of MMP-2 and MMP-9 in fibroblasts from CDF. Conclusion The reason that CDF was difficult to be cured were related to significant increase of IL-1?.The increase of IL-1? not only inhibits the fibroblast proliferation,but also promotes the activity and protein expression of MMP-2 & MMP-9,and it results in the increase of extracellular matrix degradation.
