Change of Endothelial Progenitor Cells in Hemodialysis Patients and its Relation with Framingham Risk Score
- VernacularTitle:长期血液透析慢性肾功能衰竭患者内皮祖细胞的变化及其与Framingham危险评分的关系
- Author:
Ying-Xin ZHANG
;
Shuang-Feng CHEN
;
Feng-Ju WANG
- Publication Type:Journal Article
- Keywords:
Endothelial progenitor cell;
Hemodialysis;
Chronic renal failure;
Framingham risk score
- From:
Chinese Journal of Prevention and Control of Chronic Diseases
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the number and activity changes of endothelial progenitor cells(EPCs) in hemodialysis patients,and explore its correlation with the risk factors of coronary heart disease.Methords Total mononuclear ceils(MNCs) were isolated from peripheral blood of patients with chronic renal failure in long-term hemodialysis and from a normal control group by Ficoll density gradient centrifugation and then were plated on humanfibronectin-coated dishes.Mter 7 days of culture,EPCs were characterized as adherent cells by double staining with FITC-UEA-I and DI-LDL,and were further identified by the expression of CD34,CD133 and KDR with flow cytometry.EPCs migration was determined with modified Boyden chamber assay.EPCs adhesive assay was performed by replanting EPCs on humanfibronectin-coated plates and then counting the adherent cells.The relationship of the EPCs' number and activity with Framingham Risk Score of ten years was also be assessed.Results Number of EPCs and the migratory & adhesive capacity were significantly lower in patients than in the control(P
