Effects of N,N'-dinitrosopiperazine on cell proliferation in nasal and nasopharyngeal epithelia and expression levels of TRAF2 and p16 genes in transgenic mice
- VernacularTitle:二亚硝基哌嗪对转基因小鼠TNF受体相关因子2和p16的表达
- Author:
Yingchun HE
;
Daofa TIAN
;
Fangguo LU
;
Jieqiong JIANG
;
Anyi HE
;
Yan CHEN
- Publication Type:Journal Article
- Keywords:
Nasal Cavity;
Nasopharynx;
Precancerous Condition;
Piperazines;
TNF receptor-Associated Factor 2;
p16,Gene;
Mice
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of N,N'-Dinitrosopiperazine (DNP) on the cell proliferation of nasal and nasopharyngeal epithelia and the expression levels of TRAF2 and p16 genes in TgN (p53mt-LMP1)/HT transgenic mice and the relationships between them.METHODS The epithelial proliferating characteristics of nasal cavity and nasopharynx in TgN (p53mt-LMP1)/HT cancerous lesion inducing group treated by DNP (TI),TgN (p53mt-LMP1)/HT controlling group (TC), C57BL/6J cancerous lesion inducing group (CI) treated by DNP and C57BL/6J controlling group (CC) were observed for pathological evaluation by HE staining,and the expression levels of TRAF2 and p16 genes in these tissue samples were determined by immunohistochemical methods.RESULTS The occurring rates of precancerous lesions in nasal and/or nasopharyngeal epithelia in TI,TC,CI and CC groups were 90%,10%,0 and 0,respectively (P
