Expression and assaying of thymic stromal lymphopoietin in nasal mucosa of murine model of allergic rhinitis
- VernacularTitle:胸腺基质淋巴细胞生成素在变应性鼻炎小鼠鼻黏膜中的表达
- Author:
Xu LI
;
Jun CHEN
;
Jianhua QIU
;
Li QIAO
;
Xiujie CHUNYU
;
Ying LIN
- Publication Type:Journal Article
- Keywords:
Thymopoietins;
Rhinitis,Allergic,Perennial;
Models,Animal
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the content and distribution characteristics of thymic stromal lymphopoietin (TSLP) in nasal mucosa of murine model of allergic rhinitis.METHODS 6-week-old BALB/c mice were divided into control group and experimental group.Mice of experimental group were sensitized by means of dropping OVA in nasal cavity and intraperitoneal injection of OVA. Meanwhile,mice of control group were dealt with physiological saline instead of OVA.The content of TSLP in nasal mucosa of mice was tested by means of real-time PCR and its distribution was detected by means of immunohistochemistry staining.RESULTS The content of TSLP was obviously higher in nasal mucosa of experimental group than that of control group.It was distributed mainly in lamina propria and on the surface of epithelium.CONCLUSION TSLP may play an important role in allergic rhinitis of the mice.
