Effect of Haikun Shenxi Capsule on Endothelin-1 and mRNA Expression in Kidney Tissue of Rats with Adriamycin-induced Nephrosis and Proliferation of Glomerular Mesanglal Cells
- VernacularTitle:海昆肾喜对阿霉素肾病大鼠肾组织内皮素1及其mRNA表达和肾小球系膜细胞增殖的影响
- Author:
Kun LIU
;
Zong-Jiang ZHAO
;
Xin-Xue ZHANG
;
- Publication Type:Journal Article
- Keywords:
Haikun Shenxi Capsule;
Adriamycin-induced nephrosis;
Rats;
Endothelin-1(ET-1);
Proliferation
- From:
Journal of Traditional Chinese Medicine
1993;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Haikun Shenxi Capsule(HSC)on endothelin-1(ET-1)and mRNA expression of rats with adriamyein-induced nephrosis and proliferation of glomerular mesangial cells(GMCs)cultured in vitro.Methods The rats were randomized into 6 groups,normal group,sham operation group,model group,benazepril group,HSC low-and high-dose groups.Ten weeks after treatment,ET-1 and mRNA expression were measured with immunohistochemical and hybridization in situ method.Tak- ing the Benazepril group as the control group,the proliferation of GMCs was detected with MTT method.Results In the model group,ET-1 and mRNA expression presented a strong positive,compared with which,that of the HSC low-and high-dose groups was remarkably reduced(P
