Study of the gene expression profiles in human allergic rhinitis
- VernacularTitle:人类变应性鼻炎基因表达谱的研究
- Author:
Bing LIU
;
Jian WU
;
Shuwei ZHAO
;
Jingping FAN
;
Aihua SUN
;
- Publication Type:Journal Article
- Keywords:
Gene Expression Profiling;
Rhinitis,Allergic,Perennial;
Oligonucleotide Array Sequence Analysis;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the changes in gene expression in patients with allergic rhinitis(AR). METHODS 14500 gene DNA microarray(Affymetrix) was used to examine the gene expression in 6 AR nasal mucosal samples and 6 normal nasal mucosal samples.The differentially expressed genes were identified and subjected to real time RT-PCR analysis. RESULTS Compared with normal sinus tissues,161 genes were found to be differentially expressed in AR samples.Forty-seven genes were upregulated,114 genes were downregulated.The differentially expressed genes mostly involved in immune transcription regulatory molecules,signal transduction.Real-time RT-PCR results of CCL20,GPK4 were consistent with that of gene chip analysis.CONCLUSION Microarray expression profile of AR samples is differential.GPK4 and CCL20 may play an important role in cell signal transduction in mechanisms of AR.
