Antitumor activity of Paecilomyces tenuipes polysaccharide and its mechanism in vitro
- VernacularTitle:细脚拟青霉多糖体外抗肿瘤作用及其机制的实验研究
- Author:
Jiang-Cheng ZUO
;
Jian-Xin LV
;
Li-Qin JIN
;
Li-Lin ZOU
;
Dong LI
;
Zhen-Huan MING
- Publication Type:Journal Article
- Keywords:
Paecilomyces;
Polysaccharides;
Antitumor activity;
Immunity
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the antitumor activity and mechanism of Paecilomyces tenuipes polysaccharide(PTPS).METHODS: PTPS-I was obtained by water extraction and alcohol precipitation,and purified by DEAE-cellulose and Sephadex G-100 chromatography.Human erythroleukemia cell line K562,laryngocarcinoma cell line Hep2 and hepatic carcinoma cell line SMMC-7721were co-cultured with PTPS-I or the conditioned medium which prepared with PTPS-I-stimulated human mononuclear cells(PTPS-I-MNC-CM),and the proliferation of tumor cells was determined.The cell counting kit-8(CCK-8) was used to determine the proliferation of MNCs.The FQ-RT-PCR was applied to investigate the expression of TNF-? and IL-6 mRNA in MNCs.RESULTS: PTPS-I-MNC-CM inhibited the proliferation of K562,Hep2 and SMMC-7721 cells in vitro(P
