VIesenteric lymph of shock rats activates peripheral neutrophils
- VernacularTitle:失血性休克大鼠肠系膜淋巴液对中性粒细胞的活化作用
- Author:
Li-Bo LI
;
Xiu-Jun CAI
;
Wen-Zhi XU
;
Ming-Hui LIU
;
- Publication Type:Journal Article
- Keywords:
Shock;
Wounds and injuries;
Animal experimentation;
Lymph;
Granulocytes
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the peripheral neutrophils activation mesenteric lymph in a murine hemorrhagic shock model.Methods In this study,18 male Sprague-Dawley rats were evenly divided into 3 groups.Group A:rats subjected to hemorrhagic-shock and Ringer's lactate(RL)resuscitation,group B:rats suffered from no blood loss but received same amount of RL as in group A,and group C:rats experience no blood loss nor RL transfusion.The main mesenteric lymphatic duct was cannulated with 24G catheter in all rats.In group A,blood was withdrawn through femoral artery until mean arterial pressure reached 40?5 mm Hg,the pressure was maintained for 90 min.In group B,no blood was withdrawn,these two groups received RL 3 times as the blood withdrawn,in group C,no blood was withdrawn,nor fluid was given.Lymph samples during pre-shock,the first hour and second hour post-shock or sham shock were collected and was used to induce PMN activation.Mesenteric lymph-induced rat PMN(polymorphonuclear neutrophil)adhesion molecule expression(CD18 and CD11b)and neutrophil respiratory burst activity was examined using a FACS flow cytometer.Results In group A,1st hour and 2 nd hour post-shock mesenteric lymph induced rat PMN activation,the expression of CD11b was 63.28?1.13%,61.23? 1.16%,respectively,compared with control(P
