Effects of Rosiglitazone on Transforming Growth Factor-?1/SMADs Signal Pathway in Diabetic Rat Myocardium and Cardiac Remodeling
- VernacularTitle:罗格列酮对糖尿病大鼠心脏转化生长因子-?1/SMADs信号转导通路及心脏重塑的影响
- Author:
Longying LI
;
Qian XIAO
;
Yuan GAO
;
Aibin GAO
;
Qinan WU
;
- Publication Type:Journal Article
- Keywords:
Diabetic cardiomyopathy;
SMADs;
Rosiglitazone
- From:
Chinese Circulation Journal
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of rosiglitazone on transforming growth factor-?1(TGF-?1)/SMADs signal pathway in dia- betic rat myoeardium(DM)and cardiac remodeling. Methods:Thirty male SD rats were randomly divided into normal control,DM and rosiglitazone groups,each having 10 rats.Diabetic rats were induced by a single intrapefitoneal injection of streptozotoein(60mg/kg).A cannula connected to a trans- ducer was inserted into the heart to measure the cardiac function.The body weight,heart weight and heart weight/body weight (HW/BW)were measured.The ultrastruetural changes were evaluated by electron microscope and collagen content was assessed by Van Gieson staining.The mRNA expression level of SMAD3 and SMAD7 were determined by RT-PCR.The protein level of TGF-?1,SMAD3 and SMAD7 were detected by immunohistochemistry. Results:Compared with the normal controls,diabetic rats experienced marked myocardium damage.Cardiac function,espe- cially diastolic function was impaired,HW/BW(P
