Effects of 1320 nm non-ablative laser on the proliferation of human dermal fibroblasts and the secretion of basic fibroblast growth factor and transforming growth factor-?1
- VernacularTitle:1320nm激光对成纤维细胞增殖和分泌碱性成纤维细胞生长因子及转化生长因子?1的影响
- Author:
Zhen-Xiao ZHENG
;
Ai-E XU
;
Xiao-Dong WEI
;
Hao CHENG
;
- Publication Type:Journal Article
- Keywords:
Fibroblasts;
Laser;
Transforming growth factor beta;
Fibroblast growth factor 2
- From:
Chinese Journal of Dermatology
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of 1320 nm non-ablative laser on the proliferation of human dermal fibroblasts,and the secretion of basic fibroblast growth factor(bFGF)and transforming growth factor-?1(TGF-?1)in vitro.Methods Human dermal fibroblasts were cultured,and irradiated three times by 1320 nm laser at a dose of 15,20 and 24 J/cm~2,respectively.The levels of bFGF and TGF-?1 were examined by ELISA at 0,24,48 and 72h after the irradiation.The number of fibroblasts before and after irradiation were determined.Results The number of fibroblasts and the secretion of bFGF both in- creased after the irradiation at the doses of 20 J/cm~2 and 24 J/cm~2(P
