Effects of intense pulse light on the content of collagens and mRNA expression of procollagen in BALB/c mouse skin
- VernacularTitle:强脉冲光对BALB/c小鼠皮肤胶原的影响
- Author:
Yan CAO
;
Di WU
;
Dan LUO
;
Jing XU
;
Li-Xian XU
;
Bin CHEN
- Publication Type:Journal Article
- Keywords:
Procollagen;
Collagen typeⅠ;
Collagen typeⅢ;
Mice,inbred BALB C;
Intense pulse light
- From:
Chinese Journal of Dermatology
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of intense pulse light(IPL)on the content of colla- gens and mRNA expression of procollagen in BALB/c mouse skin.Methods The BALB/c mice dorsal skin was irradiated by IPL.Skin specimens were taken at different time points after irradiation.Histopatho- logical changes were observed by hematoxylin-eosin-staining,quantitative assessment of collagenⅠand collagenⅢwas performed with immunohistochemical staining,and mRNA expression levels of procollagen were de- tected by RT-PCR.Results After the irradiation,no obvious change was observed for the staining intensity of collegen at 1 week;however,the thickening of dermis began at 2 week,and continued until 8 week.The staining of collagenⅠandⅢwas also stronger in IPL-irradiated regions than in sham-irradiated areas at 2 week(P
