Detection of serum antibodies against flagellin with a protein biochip in patients with Lyme borre-liosis
- VernacularTitle:蛋白质芯片对莱姆病患者血清抗鞭毛蛋白抗体的检测分析
- Author:
Wei-Dong DU
;
Su-Min HE
;
Xue-Jun ZHANG
;
- Publication Type:Journal Article
- Keywords:
Lyme disease;
Protein array analysis;
Flagellin;
Borrelia burgdorferi
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjeCtive To investigate the value of protein biochip in the diagnosis of Lyme borreliosis. Methods The serum IgM and IgG antibodies against flagellin were detected by flagellin coated-and N-hydroxysuccinimide (NHS) modified-biochips in 82 patients with neuroborreliosis (NB),35 patients with erythema migrans (EM) and 44 normal controls.The results were compared with those from enzyme-linked immunosorbent assays (ELISA) and Western blot.Results The levels of both IgM and IgG antibodies were significantly higher in the NB patients than those in the normal controls (P
