Genotyping of K15 gene of human herpesvirus 8 in Kaposi's sarcoma
- VernacularTitle:Kaposi肉瘤组织内人类疱疹病毒8型的K15基因型研究
- Author:
De-Zhi ZHANG
;
Xiong-Ming PU
;
Wei-Dong WU
;
Ying JIN
;
- Publication Type:Journal Article
- Keywords:
Sarcoma,Kaposi;
Herpesvirus 8,human;
Gene,K15;
Alleles
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the distribution of K15 alleles of human herpesvirus 8 (HHV-8) in Kaposi's sarcoma (KS) in Xinjiang,and to investigate the relationship between clinical profiles of KS and alleles of HHV-8 K15.Methods HHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from 27 formalin-fixed,paraffin-embedded tissue specimens of KS.The HHV-8 K15 gene was amplified by nested- PCR,and sequenced for the identification of K15 allele.Results HHV-8 DNA could be detected in 22 (81.48%) out of 27 KS patients in Xinjiang.HHV-8 DNA was detected in all 4 patients with AIDS-related KS.Twenty viral strains were identified as P type,including all 4 from the AIDS-related KS patients;two strains were identified as M type,which were all from the classical KS patients.Conclusions In KS,most of HHV-8 K15 alleles are P type,and some are M type.The 4 patients with AIDS-related KS all carried P type of K15 allele.
