Influences of UVA irradiation on the cell morphology and iNOS expression of cultured human fibroblasts and HaCaT cells
- VernacularTitle:UVA对成纤维细胞和HaCaT细胞形态及诱导型一氧化氮合酶产生水平的影响
- Author:
Ji LI
;
Wei LI
;
Hong-Fu XIE
;
Ming-Liang CHEN
;
Xiang CHEN
;
Wu ZHU
- Publication Type:Journal Article
- Keywords:
Ultraviolet rays;
Fibroblasts;
Nitric oxide synthase;
HaCaT cells
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of UVA irradiation on cell morphology,quantity and expression of inducible nitric oxide synthase (iNOS) mRNA and protein in human fibroblasts versus a kerati- nocyte cell line HaCaT.Methods Human fibroblasts and HaCaT cells were cultured and irradiated by 5 J/cm~2 UVA.Then,at 24,48 and 72 h after the stimulation,the cell morphology was observed under an in- verted microscope,and iNOS mRNA and protein were measured by RT-PCR and immunohistochemistry method,respectively.Results After the irradiation,human fibroblasts showed shrinkage at the three time points,the quantities of the cells began to decrease significantly at 24 h (P
