Relationship between the expression of multidrug resistant protein genes and fluconazole resistance in clinical isolates of Candida albieans
- VernacularTitle:白念珠菌临床株多药耐药蛋白基因表达与氟康唑耐药的关系
- Author:
Xiao-Feng QIN
;
Jian-Hua WU
;
Yi HUANG
;
Yuan-Ying JIANG
;
Jun GU
;
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Fluconazole;
Genes;
MDR;
Drug resistance;
fungal
- From:
Chinese Journal of Dermatology
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between fluconazole resistance and expression of multidrug resistant protein genes,including CDR1,CDR2,MDRI.Methods The total RNA was extracted from fluconazole-resistant and -susceptible Candida albicans isolates,and cDNA was synthesized.The expression of CDR1,CDR2 and MDRl genes was then detected by quantitative real-time PCR.The?CT (threshold cycle) value was obtained by subtracting the CT value of 18S rRNA from that of the targeted gene.Results The?CT value of CDR2 was significantly lower in fluconazole-resistant isolates than in fluconazole- susceptible isolates (7.52?2.53 vs.9.28?3.15,t=2.367,P
