Effects of ketamine on the expression of N-methyl-D-aspartate receptor-1 in a rat model of focal cerebral ischemia
- VernacularTitle:氯胺酮对局灶性脑缺血损伤大鼠脑N-甲基-D-天冬氨酸受体-1表达的影响
- Author:
Peng-Bin LIU
;
Dan CHANG
;
Zheng-Chuan SONG
;
Xiaoying DING
- Publication Type:Journal Article
- Keywords:
Ketamine;
Brain ischemia;
Receptors,N-methyl-D-aspartate
- From:
Chinese Journal of Anesthesiology
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ketamine on the expression of NMDA receptor-1(NRⅠ)in a rat model of focal cerebral ischemia and the possible mechanism of the neuroprotection.Methods Forty healthy male SD rats weighing 250-290g were randomly divided into 2 group(n=20 each):groupⅠketamine and groupⅡpentobarbital.The aminals were anesthetized with intraperitoneal ketamine 60 mg?kg~(-1) in groupⅠor pentobarbital 40 mg?kg~(-1) in groupⅡ.Focal cerebral ischemia was produced by permanent middle cerebral artery occludion(MCAO).The animals were killed at 24 h and 72 h of MCAO and their brains removed for determination of infarct size,the number of living neurons in the penumbra and the expression of NRⅠprotein(immuno- histochemistry).Results The infarct size was significantly smaller;the number of living neurons in penumbra significantly larger and NRⅠexpression significantly down-regulated in ketamine group than in pentobarbital group.Conclusion Ketamine can protect the brain against ischemia through downregulation of NMDA receptor-1.
