Effects of acetylcholine chloride on intracellular free calcium concentration of cultured sweat gland epithelial cells
- VernacularTitle:氯化乙酰胆碱对培养的汗腺上皮细胞内游离钙的调节作用
- Author:
Xia LEI
;
Jin-Jin WU
;
Yuan-Gang LU
;
Tang-You ZHU
;
Zai-Yun LONG
;
Yuan LIU
- Publication Type:Journal Article
- Keywords:
Sweat glands;
Calcium;
Acetylcholine
- From:
Chinese Journal of Dermatology
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and culture sweat gland epithelial cells in vitro,and to study the effects of acetylcholine (ACh) on intracellular flee calcium concentration ([Ca~(2+)]i) of cultured sweat gland epithelial cells.Methods Sweat glands epithelial cells were collected by enzymatic digestion.After ACh was added to the primary and first passage cells,[Ca~(2+)]i was examined using confocal laser scanning microscopy (CLSM) and the Ca~(2+) sensitive dye Fura 3/AM.Results The primary and first passage epithe- lial cells grew well.After ACh was added,opening of the calcium channel and significant [Ca~(2+)]i increase were observed when the primary and first passage cells were incubated with high concentration of calcium (2 mmol/L);no significant [Ca~(2+)]i increase was observed in those cultured without calcium.Conclusion Upon stimulation with ACh,calcium channels of cultured primary and first passage sweat gland epithelial cells would open,influx of extracellular Ca~(2+) occurred,which resulted in an increase of [Ca~(2+)]i.Extracellular bound calcium was therefore converted into intracellular free calcium.
