Construction and Expression of Plasmid Coexpressing Human Papillomavirus Type 11 E7 and Human IFN?-2b
- VernacularTitle:HPV11-E7与人干扰素?-2b融合表达质粒的构建及表达
- Author:
Wen-Zhong XIANG
;
Fei WANG
;
Guang-Ju LI
;
Xin-Jun WANG
;
Qun WANG
;
Feng LIU
;
Zhao-Song ZHANG
;
Zhi-gang Bi
- Publication Type:Journal Article
- Keywords:
Condyloma accuminatum;
Human papillomavirus;
E7 gene;
IFN伪-2b;
Fusion gene
- From:
Chinese Journal of Dermatology
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct an expression plasmid of human papillomavirus type 11 E7 (HPV11-E7)/hurnan IFN?-2b fusion gene, to express the fusion gene in E.coli BL21, and pave way for further immunological study. Methods The recombinant plasmid was introduced into E.coli BL21, then the expression product was analyzed by SDS-PAGE and Western blotting after induction with isopropy-?-D-thiogalactoside (IPTG). Results The fusion gene of HPV11-E7 and human IFN?-2b was successfully cloned into pET-32a by a linker with the same sequence as we expected. The expressed fusion protein was confirmed by SDS-PAGE and Western blotting. Conclusions The successful construction of prokaryotic expression plasmid and expression of HPV11-E7/human IFN?-2b fusion gene enable further immunological study.
