Effects of Huanglian Jiedu Decoction on IRS-1 Expression and Tyrosine Phosphorylation Level in Skeletal Muscles of the Rats with Insulin Resistance
- VernacularTitle:黄连解毒汤对胰岛素抵抗大鼠骨骼肌 IRS-1表达及酪氨酸磷酸化水平的影响
- Author:
Aili YE
;
Fu'Er LU
;
Lijun XU
;
Laibiao DING
;
- Publication Type:Journal Article
- Keywords:
Protein tyrosine phosphatase/blood;
Drug-resistance of insulin/TCM therapy;
Huanglian Jiedu Decoction/treatment application
- From:Journal of Traditional Chinese Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe effects of Huanglian Jiedu Decoction on expression of insulin receptor substrate 1(IRS-1)and the level of tyrosine phosphorylation in skeletal muscles to explore the molecular mechanism in improving insulin resistance.Methods:Wistar system insulin resistance model rats were developed with injection of small dose of streptozotocin into the caudal vein plus feeding by forage with high sugar and high lipids,and they were treated with Huanglian Jiedu Decoction for 10 weeks.Then blood sugar and serum insulin level were determined,and the changes of IRS-1 protein expression and tyrosine phosphorylation level in the skeletal muscles of the rat with insulin resistence after treatment of Huanglian Jiedu Decoction were detected with immuno- precipitation and Western Blot methods.Results:After treatment with Huanlian Jiedu Decoction,IRS-1 expression and IRS-1 tyrosine phosphorylation level in the muscular tissue increased as compared with those of the model group.Conclusion Huanglian Jiedu Decoction improves IRS-1 protein expression and tyrosine phosphorylation level in the skeletal muscle tissue of the rats with insulin resistance,which is possibly one of the mechanisms of decreasing blood sugar and improving sensitivity of insulin in tissues.
