Identification of the Polyreactivity of Monoclonal Natural Anti-ker atin Autoantibody 3B4 and Analysis of Its Molecular Mechanism
- VernacularTitle:天然抗角蛋白单克隆抗体3B4的多反应性鉴定及其分子机制的初步探讨
- Author:
Hai-Hong GUAN
;
Meng FU
;
Ru-Shan XIA
;
Gang WANG
;
Yu-Feng LIU
;
- Publication Type:Journal Article
- Keywords:
Autoantibodies;
Keratin;
Antibody specificity
- From:
Chinese Journal of Dermatology
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the polyreactivity of a monoclonal natural anti-keratin autoantibody 3B4 and to analyze its possible molecular me chanism.Methods enzyme-linked immunosorbent assay (ELISA)and immunohistoche mistry were applied to test the binding reactivity of 3B4 against different anti gens and tissues.The variable region genes and their amino acid composition wer e sequenced.Results 3B4 could reacted with a range of antigens and tissues,i n addition to keratin and skin.The variable region genes of its light chain and heavy chain showed high homology with germline genes VK1 am4 and VH1 J558.42.H CDR3 region,which mainly composed of short side chain amino acids(from 294 to 324 nucleotides around the heavy chain),was the only motif that differs from ot her highly homologous immunoglobulin genes.Conclusions The monoclonal natural anti-keratin autoantibody 3B4,with its variable region genes highly homologo us to germline genes,is highly polyreactive.The flexibility of HCDR3 may contr ibute to the polyreactivity.
